[Federal Register Volume 82, Number 93 (Tuesday, May 16, 2017)]
[Notices]
[Page 22553]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 2017-09791]
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DEPARTMENT OF HEALTH AND HUMAN SERVICES
National Institutes of Health
Government-Owned Invention; Availability for Licensing
AGENCY: National Institutes of Health, HHS.
ACTION: Notice.
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SUMMARY: The inventions listed below are owned by an agency of the U.S.
Government.
FOR FURTHER INFORMATION CONTACT: Licensing information may be obtained
by emailing the indicated licensing contact at the National Heart,
Lung, and Blood, Office of Technology Transfer and Development Office
of Technology Transfer, 31 Center Drive Room 4A29, MSC 2479, Bethesda,
MD 20892-2479; telephone: 301-402-5579. A signed Confidential
Disclosure Agreement may be required to receive any unpublished
information.
SUPPLEMENTARY INFORMATION: The following inventions are available for
licensing in accordance with 35 U.S.C. 209 and 37 CFR part 404 to
achieve expeditious commercialization of results of federally-funded
research and development. Technology description follows.
Efficient mRNA-Based Genetic Engineering of Human NK Cells With High-
Affinity CD16 and CCR7
Description of Technology: A highly efficient method to genetically
modify natural killer (NK) cells to induce expression of high affinity
CD16 (HA-CD16) through mRNA electroporation, to potentiate NK cell-
mediated antibody-dependent cellular cytotoxicity (ADCC). ADCC is
mediated by CD16\+\ NK cells following adoptive NK cell transfer, but
most humans express CD16 which has a relatively low affinity for IgG1
antibodies. However, a single nucleotide polymorphism (SNP rs396991) in
the CD16 gene, resulting in an amino acid substitution at position 158
(F158V), is associated with substantially higher affinity and superior
NK cell-mediated ADCC than those with the 158F genotype. This HA-CD16-
158V polymorphism has also been linked to enhanced ADCC capacity in
vivo. The nearly 100% efficiency of our method resulted in: (a)
Sustained surface expression of transgenes at high levels for up to 4
days without compromising NK cell cytotoxicity and viability; and (b)
augmented ADCC against Daratumumab coated multiple myeloma cells by ex
vivo expanded NK cells electroporated with mRNA coding for HA-CD16.
This system is GMP compliant and has been used previously in FDA
approved clinical trials.
Potential Commercial Applications: Infusion of a large number of
highly cytotoxic autologous ex vivo expanded NK cells expressing high-
affinity CD16 into patients, to induce a more profound anti-malignancy
response to specific monoclonal antibodies, including: multiple myeloma
(Daratumumab); lymphoma (Rituximab); breast cancer (Trastuzumab); and
colon cancer (Cetuximab).
Development Stage: Early-stage; In vitro data available.
Inventors: Richard W. Childs and Mattias Carlsten (NHLBI).
Publications:
(1) Carlsten M, Levy E, Karambelkar A, Li L, Reger R, Berg M,
Peshwa MV and Childs RW (2016) Efficient mRNA-Based Genetic Engineering
of Human NK Cells with High-Affinity CD16 and CCR7 Augments Rituximab-
Induced ADCC against Lymphoma and Targets NK Cell Migration toward the
Lymph Node-Associated Chemokine CCL19. Front. Immunol. 7:105. doi:
10.3389/fimmu.2016.00105.
(2) Carlsten M and Childs RW (2015) Genetic manipulation of NK
cells for cancer immunotherapy: techniques and clinical implications.
Front. Immunol. 6:266. doi: 10.3389/fimmu.2015.00266.
Intellectual Property: NIH Reference No. E-036-2015/0,1--US
Application No. 62/079,975, filed 14 Nov 2014; and PCT Application No.
PCT/US2015/060646, filed 13 Nov 2015.
Licensing Contact: Cristina Thalhammer-Reyero, Ph.D., M.B.A.; 301-
435-4507; [email protected].
Dated: May 4, 2017.
Cristina Thalhammer-Reyero,
Senior Licensing and Patenting Manager, Office of Technology Transfer
and Development, National Heart, Lung, and Blood Institute.
[FR Doc. 2017-09791 Filed 5-15-17; 8:45 am]
BILLING CODE 4140-01-P